ATP Bioluminescence Testing

Pharmetric Laboratory uses ATP Bioluminescence for rapid detection of microbiological contaminants in compounded sterile preparations.

Start testing today

Our Process

Light-producing living organisms are widespread in nature and from diverse origins. The process of light emission from organisms is called bioluminescence and represents a chemical conversion of energy into light. Since the work of William D McElroy showing that ATP is a limiting and key factor of the bioluminescent reaction, research has lead to a better understanding of how light is produced by fireflies (McElroy, 1947; McElroy, 1951; McElroy et al., 1953). The bioluminescence mechanism involving Luciferase enzyme is a multistep process which mainly requires Luciferin substrate, Oxygen (O2), Magnesium cation (Mg++) and ATP (DeLuca & McElroy, 1974; McElroy et al., 1953; Seliger, 1989).

ATPbioluminescence using luciferine/luciferase relies on luciferine oxidation by the luciferase and the integrated light intensity is directly proportional to ATP contents. Luciferase converts in presence of ATP and Magnesium firefly D luciferin into the corresponding enzyme-bound luciferil adenylate. The luciferil adenylate complex is then the substrate of the subsequent oxidative reaction leading to oxyluciferin. The light emission is a consequence of a rapid loss of energy of the oxyluciferine molecule from an excited state to a stable one.

1/ D-luciferin + luciferase + ATP (add Mg+) — Luciferil adenylate complex +PPi 2/ Luciferil adenylate complex (add Oxygen) — Oxyluciferin + AMP+ CO2 + light

Why choose us?

  • Significant Cost Savings
  • Increased Inventory Turns
  • Less Waste
  • Enhanced Revenue Streams
  • ATP Bioluminescence Testing
  • Compendial (USP <71>) Testing
  • Endotoxin (USP <85>) Testing

Start testing today

Save Time Testing with our
Rapid Detection Process.

Rapid testing results

Pharmetric Laboratory uses ATP Bioluminescence for rapid detection of microbiological contaminants in compounded sterile preparations

Stay usp compliant

Meet the requirements of USP while harnessing the cutting-edge technology that allows for cost savings and speeds up the release of sterile preparations without sacrificing safety or reliability.

Access your testing

Gain quick access to all of your test & data anytime, anywhere. With our website you can submit a test with only a few clicks!